PandemieGrippaleV1, Main, Exploration, bibRecord, 001783

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Identifieur interne : 001783 ( Main/Exploration ); précédent : 001782; suivant : 001784

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Auteurs : Rebecca L. Vanders [Australie] ; Peter G. Gibson [Australie] ; Vanessa E. Murphy [Australie] ; Peter A. B. Wark [Australie]

Source :

The Journal of infectious diseases [ 0022-1899 ] ; 2013.

RBID : Pascal:13-0310021

Descripteurs français

Pascal (Inist)
- Homme, Cellule dendritique, Lymphocyte T CD8, Gestation, Femelle, Femme, Phénotype, Infection, Virus grippal A(H1N1).
Wicri :
- topic : Homme, Femme.

English descriptors

KwdEn :
- CD8 T lymphocyte, Dendritic cell, Female, Human, Infection, Influenzavirus A(H1N1), Phenotype, Pregnancy, Woman.

Abstract

Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303⁺/CD1c^- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.

Affiliations:

Australie

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD8 T lymphocyte</term>
<term>Dendritic cell</term>
<term>Female</term>
<term>Human</term>
<term>Infection</term>
<term>Influenzavirus A(H1N1)</term>
<term>Phenotype</term>
<term>Pregnancy</term>
<term>Woman</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Cellule dendritique</term>
<term>Lymphocyte T CD8</term>
<term>Gestation</term>
<term>Femelle</term>
<term>Femme</term>
<term>Phénotype</term>
<term>Infection</term>
<term>Virus grippal A(H1N1)</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
<term>Femme</term>
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<front><div type="abstract" xml:lang="en">Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
 PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name>
</noRegion>
<name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name>
<name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name>
<name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name>
<name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name>
<name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name>
<name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name>
<name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name>
</country>
</tree>
</affiliations>
</record>

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Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri